Oferta en Instituto Curie: Pre-doctoral engineer position in the academic research group of Dr. P. Benaroch

Pre-doctoral engineer position in the academic research group of Dr. P. Benaroch dedicated to the study of the spatio-temporal regulation of TLR3 signaling by its molecular chaperone UNC93B1.

The molecular chaperone UNC93B1 interacts with several TLRs but specifically regulates each of them by different mechanisms. Once engaged by their ligands, TLRs elicit a strong immune activation which can become detrimental if not properly controlled. TLR3 belongs to the family of endosomal TLRs, is specific for double-stranded RNA and can elicit very strong inflammatory responses. Trying to mobilize this pathway for antitumoral purpose, TLR3 ligands are currently used in more the 50 clinical trials, but TLR3 trafficking and regulation remains in large part obscure.

UNC93B1 appears as a key player in inducing efficient compartmentalization of its associated TLRs and allowing signaling to take place only in endosomes. Ongoing work in the group focuses on unraveling the role of UNC93B1 in the TLR3 pathway which remains poorly characterized. A proteomic analysis of UNC93B1 immuno-precipitates has revealed several associated enzymes and post-translational modifications of UNC93B1. The group has been characterizing the role of these enzymes and modifications on the TLR3 pathway. We are now studying the intracellular trafficking of the various players in order to decipher how TLR compartmentalization is achieved and what is the temporal order of events.

The engineer pre-PhD position is opened in the group of Dr. P. Benaroch, in the department of Immunology headed by Sebastian Amigorena (Inserm unit U932). All groups work in a collaborative manner to encourage innovative projects involving complementary expertise. The candidate will benefit from the high-level scientific environment of the research center of Institut Curie, as well as state-of-the-art technological platforms. The lab is located in a building devoted to cell biology within the Institut Curie campus in the center of Paris. It is part of a larger campus, which can provide complementary expertise and equipment in biology as well as other scientific domains (chemistry, physics, bioinformatics).

We are looking for a motivated candidate, qualified in immunology or cell biology, and willing after a first year in our lab, to eventually continue with a PhD program. The candidate should have experience in laboratory. Expertise in biochemistry (western blots, ip etc), cellular biology (confocal microscopy etc), molecular biology (cloning and gene editing) and/or Flow cytometry will be a plus.  Written and spoken English is mandatory.

We propose an engineer contract of one-year that could be extended with a PhD period of minimum 3 years.

To apply, please submit your CV, a letter of motivation and two letters of recommendation with contact details to Philippe Benaroch:  philippe.benaroch@curie.fr

Fecha publicación: 09 diciembre 2019